Christian vocation coursework Essay

Describe how a Christian may follow the call to discipleship through daily life and work Vocation is the idea that people get a call from god to do a certain job this can also be said about being called into Christianity by god, it may not be an actual call or a big flash of light but just s feeling that you have created by god. ‘Vocation’ comes from the Latin word meaning ‘to be called’. Some Christians may say that they were called to Christianity and so were all Christians. For example Jesus was called by god, he heard his voice when he was baptised by John. Discipleship is another term used my Christians used to mean following Jesus like his disciples did, living a Christian lifestyle and helping others also apply to discipleship. Christians follow Jesus by following his examples like his love, service and compassion towards others. Someone who has chosen to follow Jesus should be living a caring helpful life, possibly in a caring profession such as a doctor, nurse or teacher. Any job can be a vocation, some Christians say god has a plan for everyone and he may call you to a job that isn’t related to Christianity at all. St. Paul said in Colossians 3:23 â€Å"whatever you do, work at it with all your heart, as working for the lord, not for men.† Possibly meaning that god has called everyone to their jobs because he knows its right for them so work for him and do the job well. A Christian may choose his or her job just simply because they want to do the job although this want or urge to do a job could be put down to god. I don’t think there are any jobs unsuitable for Christians, Jesus loved everyone anyway even if they were a sinner he did not commend what they did but he still loved them. I do not think your religion should constrain your job opportunities. A Christian may serve god in an ordinary job by obeying the 10 commandments in their job and generally being caring and helpful. Christians can put Christian teachings into their family lives by following Paul’s teachings and the 10 commandments. Paul’s teachings say, â€Å"A wife, must put her husband first. This is her duty as a follower of the lord. A husband must love his wife and not abuse her. Children must always obey their parents. This pleases the lord. Parents, don’t be hard on your children. If you are, they might give up. (a)(ii) Explain why some Christians join communities and take vows or poverty, chastity and obedience. Christian communities are a group of Christians who live together. A convent is where a group of female Christians would live also known as nuns and male Christians or monks would live in a monastery. A contemplative order is focusing your life on god, in prayer and everyday life. An example of a contemplative community is the poor clares. The normal day in the poor clares community may consist of many things, gardening, housework, cooking, cleaning, helping the elderly and sick, praying or working in the shop; making candles, encaustic wax cards, as well as cards created and printed on a computer, turning wood and knitting garment on their knitting machine. The nuns do this type of work involving their hands because it leaves their minds free to focus on god and pray. Jesus says in Matthew 6:24 how â€Å"no one can serve two masters†, â€Å"you cannot serve both god any money† so the nuns in the poor clare community decide to â€Å"serve god† and his people and help them rather than work for money. An active order is doing something about what you believe in, for example if you want to be one of Jesus’ disciples you would follow in his footsteps and his examples and help people maybe by helping out at a hospital or a school. People or monasteries and convents today are living like the first Christians as recorded in the book of acts because the first Christians lived out alone in the desert, Christians live in s slightly similar way today. They have no possessions, they cannot have sex and they dedicate their time to thinking if they are contemplative, as you would alone in a desert, or doing things to help others. Living in a Christian community would help a Christian to follow their call to discipleship because of the vows they take when they join, they have to do things in a similar way to Jesus therefore helping to follow their call to discipleship. Some Christians may say joining a Christian community is a vocation because whatever they do as a job or joining a community is a vocation. God has a plan for them and they follow it via vocation. Some Christians believe that god want them to live in a religious community rather than an ordinary one because its better for them and other who they would be helping. A vow is an oath or promise you make. Nuns and monks take vows when they join a religious community; they take a vow of poverty so they are not tied down by possessions and money and can focus on god. Christ was poor to make us rich so they follow in his footsteps and enforce discipleship. They also take a vow of chastity, which means they must remain celibate so they can devote themselves to god as Jesus did. Also a relationship with someone from the opposite sex will get in the way of god and praying; St. Paul advises Christians to be celibate so they can â€Å"be devoted to the lord†. Obedience is the third vow they take, by this vow they give to God their freewill. They must be obedient to their abbot or abbess. Again showing their discipleship, because Jesus was obedient until the point of death on the cross for god. It is important for Christians to make these vows in front of a community because then there is witnesses so you cant get out of the commitment and also if you are doubtful they can remind you about them. (a)(iii) Explain the purpose and practice of one particular religious community. The poor Clares are a religious community for women who want to dedicate their lives to god. They originated in 1212 by St Francis and St Clare in Assisi in Italy. The poor clares are a contemplative community. The contemplative community of nuns started as a way of being united with god and to follow Jesus. The aims of the poor Clare community are mostly â€Å"to pray for the world and the Church†, to live a life through their prayers, dedicated to god and â€Å"to live out the Gospel command of love† which means to live their lives as god wanted helping others and loving them. The normal day in the poor clares community may consist of many things, gardening, housework, cooking, cleaning, helping the elderly and sick, praying or working in the shop; making candles, encaustic wax cards, as well as cards created and printed on a computer, turning wood and knitting garment on their knitting machine. The nuns do this type of work involving their hands because it leaves their minds free to focus on god and pray. Jesus says in Matthew 6:24 how â€Å"no one can serve two masters†, â€Å"you cannot serve both god any money† so the nuns in the poor clare community decide to â€Å"serve god† and his people and help them rather than work for money. Their timetable is shown below 5.00 Rise 5.45 Readings – a morning invitation to prayer composed of some psalms and two readings, one from Scripture and one from a Christian teacher or saint. These nourish our spirits for the day ahead. A quick breakfast followed by time for personal prayer, reading, study until 7.30 7.30 Morning Prayer, the Prayer of Praise to God followed by Spiritual reading 8.30 Holy Mass followed by a short Office and then work until 11.50 11.50 A short Prayer written by Francis of Assisi and Scripture reading 12.30 Dinner; some free time until 2.15 2.15 back to work with a tea break at 3.30 and a short prayer together in the refectory at 4.00 to sanctify our work 5.50 Evening prayer 6.30 Supper followed by an hour of personal prayer time until 8.15 8.15 Compline or Night Prayer, which closes the day. The poor clares take three vows as a sign of their commitment and consecration to God. The vow of chastity allows the nuns to focus their love on god, they give god their bodies and souls and their right to marry. They also take the vow of poverty so they are not tied down by possessions and money and can focus on god. Christ was poor to make us rich so they follow in his footsteps and enforce discipleship. Obedience is the third vow they take, by this vow they give to God their freewill. Again showing their discipleship, because Jesus was obedient until the point of death on the cross for god. (b) â€Å"Christians should lead active rather than contemplative lives.† Contemplative means to life your life dedicated to god through prayer, to live a contemplative life. An example of a contemplative community is the poor clares. a day in the poor clares community may consist of things such as , gardening, housework, cooking, cleaning, helping the elderly and sick, praying or working in the shop; making candles, encaustic wax cards, as well as cards created and printed on a computer, turning wood and knitting garment on their knitting machine. The nuns do this type of work involving their hands because it leaves their minds free to focus on god and pray. Jesus says in Matthew 6:24 how â€Å"no one can serve two masters†, â€Å"you cannot serve both god any money† so the nuns in the poor clare community decide to â€Å"serve god† and his people and help them rather than work for money. Thomas Merton was a Trappist monk; he took a vow of silence amongst other vows his writings have had a big influence on many Christians. He took part in many social protests even though he had to be silent; he took part in such protests as the struggle for racial equality in the USA and the struggle against nuclear weapons in the Vietnam War. An active life for a Christian is doing something about what you believe in, for example a Christian can help follow their path to discipleship by following Jesus and helping others by helping people at hospitals, nursing homes, schools etc. ‘ordinary’ Christians can lead active lives by living in a Christian lifestyle and helping others also apply to discipleship. Christians follow Jesus by following his examples like his love, service and compassion towards others so do this in your everyday life and work and you will be living an active life. ‘Extra-ordinary’ Christian could do many great things for example Martin Luther King, Jr. (January 15, 1929 – April 4, 1968) was one of the main leaders of the American civil rights movement he was a Baptist minister, and had a huge impact on the people of America. his most influential and well-known speech is the â€Å"I Have A Dream† speech. In 1964, he became the youngest man to be awarded the Nobel Peace Prize. he spoke out against injustices against black people of america, they were not allowed to vote, not allowed in the same places as white people and got half the pay. Martin luther king changed this Another example of a ‘extra-ordinary’ active christian was Mother Teresa (August 26, 1910 – September 5, 1997), she was born a roman catholic nun, she won the nobel peace prize in 1979. she was fascinated by stories of the lives of missionaries and their service. By the early 1970s, Mother Teresa had become an international celebrity. Her fame can be partly attributed to the 1969 documentary ‘Something Beautiful for God’. In 1982, at the height of the siege in Beirut, Mother Teresa rescued 37 children trapped in a front line hospital by negotioating a temporary cease-fire between the Israeli army and Palestinian guerillas. Mother Teresa inspired a wide variety of commemorations. Besides receiving numerous honors during her lifetime, she has also been memorialized through museums, been named patroness of various churches, and had various structures and roads named after her. In conclusion living an active life is far more beneficial to others and is more noticeable, living a contemplative life is good if you believe prayers work and they really were helping. Christians serve god in many different ways some more subtle than others. However it is to be taken into consideration that active Christians do pray and contemplate and contemplative Christians do work and help others. In my opinion active Christians are making a more valuable contribution to the world because I do not believe that prayers do anything and even if god doesn’t exist active Christians are still doing something good for the world

Hcs 301 – Personal Goals

Personal Goals HCS 301 September 25, 2010 Judy Ceppaglia Personal Goals It wasn’t that long ago that I had those new graduate nurse’s hopeless feelings of fear and despair, as whether or not I was ever going to be a competent nurse. Wondering whether I was ever going to feel like I know exactly what I am doing and why. Was I ever going to stop being nervous when starting an IV or inserting a Foley catheter. Was I ever going to handle a full assignment of four patients, in busy Emergency Room (ER), without the help of a preceptor. As fast as those feelings overwhelmed me, they have disappeared.These days, the feelings of fear and despair aren’t as noticeable. â€Å"Goal setting plays a prominent role in social-cognitive learning models of academic achievement† (Morisano, Hirsh, Peterson, Pihl, & Shore, 2010). When I can accomplish my goals I feel as though the sky’s the limit. The time has finally come, for me to reach to the stars and grab one, taki ng my world by storm. According to Weber, â€Å"Every goal you set is a life goal, whether it’s a business goal, a relationship goal, a health goal, a spiritual goal, or a financial goal, every decision you make every goal you set has a personal consequence.We all know our goals need to be smart goals. Specific, measurable, achievable, results-oriented and time defined† (Weber, 2009, para. five – seven). Sometimes personal and professional goals are very similar and may even become intertwined due the outcomes and decisions made by the individual. A decision made in one area may have effects in another. At present my short professional goal is to become certified in the insertion of external jugular (EJ) IV’s.Because of the socio-economic class of patients I work with, and the patient’s presentation with almost no peripheral IV access from poor circulation, to uncontrolled illnesses, or IV drug abuse, this skill few are certified in. The entire pro cess entails taking home the hospital policy and accompanying learning packet, taking an in class test and acquiring six chaperoned insertions of EJ’s with a nurse who is already certified. My plan is to have this goal achieved by January 2011. According to McKay (n. d. ), â€Å"Short term goals are reachable in one to three years.Short term goals are often a stop along the way to our long term goals† (Planning for the future para. 3). My second professional goal being long-term in is to go from a registered nurse (RN) level II to a level III RN. The hospital that I work for offers room for advancement through a three level tier program. Starting with my basic RN license, moving toward level II and then onto level III, each advancement comes through educational and specified guidelines established by the hospital and set forth in a hospital policy.Prior to going back to school I was able to attain my level II status. However, the requirements for a level III require th e achievement of a Bachelor’s degree and certification in a specialized field. This means acquiring a Certified Emergency Nurse (CEN) certificate. I anticipate my graduation from the BSN program to be in May 2012 and then giving me six months to study for and mastering the CEN test. At that time I will obtain an updated copy of the policy and requirements and revisit the application process to becoming a level III RN.According to McKay (n. d. ), â€Å"Long term goals can take about three to five years to achieve† (Planning for the future,  para. 3), and with â€Å"Goal clarity, increases persistence, making individuals less susceptible to the undermining effects of anxiety, disappointment, and frustration (Morisano, et al. 2010, p. 256). In setting personal goals, I that I tend to struggle more with these. Even the simplest of things can be a large obstacle to overcome. This leads me to a short-term personal goal of just being able to keep up on the laundry although I am in school.So often the laundry can be one of those things that is easily over looked. In the past, I would dedicate one day of the week, usually Sunday to do all of the laundry but that became overwhelming and consumed my entire day, usually resulting in laundry being left in the dryer and a load or two not folded and properly put away. Because of that I eventually just slowly stopped doing the laundry until we, were in desperate need of something. I began having feelings of guilt and eventually I started to try to do a load here and there, but I felt that it was a never-ending battle.For these reasons I want to establish a better relationship with the laundry monster and do at least one load of laundry a day, which includes washing, drying, and putting it away. According to The New York Times Company (2010), â€Å"Today’s preparation determines tomorrow’s achievement, Author Unknown† (Quotations, para. 1). â€Å"Striving for personal goals assigns meanin g, structure and direction to an individual’s life† (Conrad, Doering, & Exner, 2009.Looking to the future, I believe that to be a good example for not only my children but my prospective students, I need to lose 55 pounds, and establish the healthier lifestyle I once had, making this my long-term personal goal. In April 2005 I had begun eating healthier and exercising that subsequently led to the loss of about 65 pounds. After being accepted to the nursing program in August 2007, I slowly lost the energy and time it took to continue eating healthy and exercising, therefore losing those good habits that were adopted as my everyday living and eventually resulted with gaining back about 50 pounds.In an article written by Wood and Neal (2007) they state, â€Å"Habits are learned dispositions to repeat past responses. They are triggered by features of the context that have covaried frequently with past performance, including performance locations, preceding actions in a seq uence, and particular people. After reading this article I found the solidification that I needed and was reminded that I can accomplish my goals. Moving forward, I want to have regained my healthier weight and exercise routine before the completion of the BSN program.In conclusion, with the establishment of clear, concise and attainable goals the work required to achieve those goals makes them easier to aspire toward. With continued success, motivation, and upward movement toward a person’s goals, the less likely they are to become unmotivated, disappointed, and frustrated. â€Å"Well defined goals appear to help individuals discover and use ever more efficient strategies and modes of thought and perception† (Morisano, et al. 2010. p. 256) References Conrad, N. , Doering, B.K. , & Exner, C. (2009,  October). Looking beyond the importance of life goals. The personal goal model of subjective well-being in neuropsychological rehabilitation. Clinical rehabilitation 2010 ; , (24), 431 – 443. McKay, D. R. (n. d. ). Goal setting – increase your chances for success. Retrieved from http://careerplanning. about. com Morisano, D. , Hirsh, J. B. , Peterson, J. B. , Pihl, R. O. , & Shore, B. M. (2010). Setting, elaborating, and reflecting on person goals improves academic performance.Journal of Applied Psychology, 95(2), 255-264. doi: 10. 1037/a0018478 The New York Times Company. (2010). About. com. Retrieved from http://quotations. about. com/cs/inspirationquotes/a/Success1. htm Weber, S. (2009, December  24). Have a plan and goals. Redland Daily Facts. Retrieved from http://proquest. umi. com. Wood, W. , & Neal, D. T. (2007). A new look at habits and the habit-goal interface. The American Psychological Association – Psychological Review, 114(4), 843-863. doi:10. 1037/0033-295X. 114. 4. 843

Mahogany Seed as a Termiticide to Kill Termites

Mahogany used in multistory systems in the Philippines, boat and ship building and patternmaking. Logs are used for the manufacture of veneers and for paneling. It is also used as shade for coffee and cacao. Mahogany is regarded as the worlds finest timber for high-class furniture and cabinetwork. Its popularity is especially due to its attractive appearance in combination with ease of working,excellent finishing qualities and dimensional stability. Mahogany is also often used for interior trim suchas paneling, doors and decorative borders.It is used for boat building, often as a decorative wood for luxury yatch and ocean liners, although it is also used when a medium-weight timber with other goodqualities is required. It is sometimes applied make it particularly suitable for precision woodwork suchas models and patterns, instrument cases, clocks, printer's block and parts of musical instruments; for these purposes, uniform straight-grained material is used. Other minor uses include burial caskets, woodcarvings, novelties, toys and turnery.BACKGROUND OF THE STUDYMahogany a large tropical tree with a symmetrical appearance, best-known for its valuableheartwood. The tree is also appreciated as a beautiful and useful street tree. A fast-growing, graceful,straight-trunked, semi-deciduous tree growing to 30-70ft. Most trees, particularly planted street treesgrow to 30-40ft. It looses its leaves just as new leafs sprout, so while deciduous, the tree is not withoutleaves for long. Tiny flowers are followed by 4-5†³, woody fruits that burst open to expel the seeds. Mahogany is a valuable hardwood and this tree was once extensively harvested for its wood.A relatedtree, S. macrophylla, now provides most commercial mahogany. The tree also makes an excellent streettree specimen in warmer climates as is popular for this purpose. Miami, Florida has numerousmahogany trees planted throughout the city for this purpose. The termites are a group of eusocial insects usually c lassified at the taxonomic rank of order Isoptera (but see also taxonomy below). Along with ants and some bees and wasps which are all placedin the separate order Hymenoptera, termites divide labour among gender lines, produce overlappinggenerations and take care of young collectively.Termites mostly feed on dead plant material, generallyin the form of wood, leaf litter, soil, or animal dung, and about 10% of the estimated 4,000 species(about 2,600 taxonomically known) are economically significant as pests that can cause seriousstructural damage to buildings, crops or plantation forests. Termites are major detritivores, particularlyin the subtropical and tropical regions, and their recycling of wood and other plant matter is of considerable ecological importance. Their role in bioturbation on the Khorat Plateau is under investigation.SIGNIFICANCE OF THE STUDYNowadays, people usually choose new innovations (features) to kill termites or any other pests. Pesticides are usually used to kill a particular target pest, many will also kill or harm species that thefarmer or other user is not targeting. For example, pesticides applied to crops might be washed intostreams or lakes and harm fish, beneficial insects, birds, or even find their way into drinking water sources. With this regard topic it includes improvement in human quality of life and lower food costs. Contributed significantly to improving the quality of life and safeguarding the environment.STATEMENT OF THE PROBLEMIt should be only used and tested in termites.B. CONCEPTUAL FRAMEWORKHYPOTHESISMahoganyseed extract Used a stermiticide tokill termitesOBSERVATORY;On Savanna, Termites Are a Force for Good By SINDYA N. BHANOOPublished: June 1, 2010The African savanna has a cornucopia of majestic creatures — lions, elephants and giraffes amongthem. But behind the scenes, it is the tiny termite that fuels much of this diversity, a new study reports. Researchers studying termites in Kenya's central highlands found that the abundance of flora andfauna is markedly higher atop termite mounds.†We noticed these circular green patches,† said Todd Palmer, a co-author of the study and a professorof biology at the University of Florida. †They had a lot of vegetation and plant material on top of them,and the grass was greener than in other areas. †The patches were 30 feet in diameter and spaced several hundred feet apart. Dr. Palmer and his colleagues did some digging, and underneath each patch they found millions of termites in subterranean mounds. Quantitatively, they found that plants grow about 60 percent largeron the patches compared with other areas.The nitrogen content of the plants on the mound is about20 percent higher, and trees on mounds bear 120 percent more fruit. Animal populations also droppedoff significantly the farther they were located from a patch. Termite mounds are rich in nutrients like nitrogen and phosphorus, and termites also help loosen soilto p romote water absorption, Dr. Palmer said. Other animals visit the lush patches to eat and end updefecating and urinating there, adding their own nutrients and triggering more plant growth. In the human world, termites are seen as pests for their remarkable ability to eat into dead wood.Butin the animal kingdom, Dr. Palmer said, this is what makes them so desirable. †They are basically consuming dead wood and plant materials,† he said. †In their absence, that would just lie there and there would be no way to break down the organic material and convert it to nitrogenand phosphorus. †How Termites Live on a Diet of Wood By NNIICCHHOOLLAASSWWAADDEE Published: November 14, 2008 If only wood could be converted tobbiioof f uueellss, there would be no need to wait a million years for thetrees to be buried and become oil. Wood isindeed convertible to useful chemicals, because termitesdo it every day, causing $1 billion of damage every year in the United States. But to live on a diet of wood is challenging, not least because wood contains so little nitrogen. So how do termites do it? Visual ScienceScientists rely on graphics and other visuals to present their findings to the world. This feature takesraw graphics from various scientific journals and unpacks the stories they tell. The trick lies in a cunning triple symbiosis, a team of Japanese scientists report in Fridays issue of Science.In the termites gut lives an amoeba-like microbe called a protist, and inside each protist livesome 10,000 members of an obscure bacterium. The microbes in the termites gut are very hard to cultivate outside their termite host and so cannot bestudied in the lab. The Japanese scientists, led by Yuichi Hongoh and Moriya Ohkuma at the RIKENAdvanced Science Institute in Saitama, have cut through this problem. They extracted the protistsbacteria directly from a termites gut, collected enough to analyze their DNA, and then decoded the1,114,206 units of DNA in the bacter iums genome. WEDNESDAY, FEBRUARY 28, 2007Entomologists discover cellulase genes in termite gutS As scientists search for alternatives to fossil fuel, producing chemical energy from wood fiber has become a big challenge. Several research organisations and biotech companies are trying to discover enzymes that break down cellulose into glucose in an efficient way (earlier post). However, termites have been working this alchemy for millions of years. A University of Florida (UF) study published last month in the journal Gene sheds new light on the mysterious and complex process that enables the insects to eat the cellulose, the main structural component of plant cells.For people and most animals, cellulose is indigestible, but termites break it down easily into glucose, a form of sugar most organisms need. These sugars can be fermented into bio-products, such as ethanol or bioplastics. The study identifies four genes that produce enzymes responsible for taking cellulose molecules apart in a process called cellulase (picture, click to enlarge) insight that could lead to breakthroughs in energy production and pest control, said Michael Scharf, an assistant research scientist with UF’s entomology department and a co-author of the paper.The scientists looked at the dominant termite species in the U. S. but they are sure they haven't identified all the genes involved in producing these enzymes yet. Only one of the genes actually belongs to the insect researchers studied, the eastern subterranean termite. The other three belong to microscopic organisms known as symbionts that live inside the termite’s digestive system: â€Å"The termites provide the symbionts with a home, and the symbionts pay the rent by producing enzymes,† says Sharf. Altogether, there may be hundreds of cellulose-digesting enzymes produced by the termites and their tiny tenants, Scharf said.One potential payoff from the research is that scientists may be able to transfer specific enzyme-producing genes into bacteria, then culture them to produce large quantities of enzymes to make ethanol from wood scraps and other fibrous materials, he said. Known as cellulosic ethanol, this fuel has gained worldwide attention because it doesn’t require edible material such as corn, used in conventional ethanol production. The interaction of multiple genes makes cellulose digestion an efficient process in termites, but scientists want to pin down enzyme combinations that will digest cellulose affordably, Scharf said.Many genes remain undiscovered, and UF researchers have applied for funding to support a massive effort to identify all cellulose-digesting genes in the eastern subterranean termite and its common symbionts. Greater genetic knowledge could also aid in termite control, an important issue in Florida, which accounts for about one-third of control efforts in the United States, said Phil Koehler, a UF entomology professor and co-author of the paper. By identi fying enzymes most crucial to termite digestion, scientists may be able to kill the insects by shutting down selected genes, he said.Termite-control strategies, such as bait systems or treated lumber, would be environmentally friendly because they would have no effect on organisms that don’t eat cellulose, he said. â€Å"Anything we do with this kind of work will reduce the need for conventional pesticides,† Koehler said. Development of enzyme-blocking products could happen but will require attention to termite behavior, said Brian Forschler, an entomology professor at the University of Georgia in Athens. Recent research shows that termites, which live in colonies that can number 1 million, often consume partially digested material excreted by their compatriots, he said.So it would be important that bait products not disrupt termites’ feeding behavior. If it did, termites might avoid an enzyme-stopping bait and instead share more partially digested food. â€Å" You just have to remember that you’re dealing with an entire termite colony,† Forschler said. â€Å"This research holds a great deal of promise. † Further termite genetics research could reveal effective methods of disrupting termite social behavior, perhaps in ways that cause the insects to die, said Faith Oi, an assistant extension scientist with UF’s entomology and nematology department.â€Å"The model for exploiting the termite’s social behavior for control is not new,† said Oi, another co-author of the paper. â€Å"In terms of pest control, we can look to this area of science enhancing existing methods. † Bed Bug Herbal Remedies Work Well With Traps July 15, 2013 THE NEEM TREE (Azadirachta indica), a medicinal mahogany tree (Meliaceae) native to arid broadleaf and scrub forests in Asia (e. g. India), has been used for over 4,000 years in Vedic medicine and has a heavy, durable wood useful for furniture and buildings because it is resistant to termites and fungi.Nonetheless, despite US EPA registration as a pesticide for crop and home use and a long legacy of neem seed oil use for cosmetics, shampoos, toothpastes and medicines in India, Ohio State University researcher Susan Jones could not find any households near her Columbus, Ohio, home willing to try neem in her bed bug control experiments. â€Å"We had no study takers because of the regulatory requirements,† which scared off people, Jones told the Entomological Society of America (ESA) Annual Meeting.â€Å"You have to read page after page to residents about toxicity without being able to talk about the toxicity of alternative products† not as safe as neem. In October 2012, an empty house with bed bugs became available for research when its occupant opted to escape a bad bed bug infestation by leaving the infested home; and inadvertently transferred the infestation to their new home. Jones monitored the empty house by placing in each room fo ur (4)Verifi(TM) CO2 (carbon dioxide) traps and four (4) Climbup(R) Interceptor traps. Visual inspections revealed few bed bugs.On October 24, 2012, prior to neem treatments, 38 bed bugs were captured in Climbup(R) traps, indicating bed bug infestations only in the master bedroom and bed of the empty house. Eight Verifi(TM) traps captured 48 bed bugs in the dining room, guest room and master bedroom. As part of an IPM (integrated pest management) approach using multiple treatment tools: Electrical sockets were treated with MotherEarth(R) D diatomaceous earth; 3. 67 gal (13. 9 l) at a rate of 1 gal/250 ft2 (3. 9 l/23 m2). Gorilla Tape(R) was used to seal around the doors and exclude bed bug movement from other rooms.The neem seed oil product, Cirkil (TM) RTU, was sprayed in various places, including on books, backs of picture frames and cardboard boxes. Vials of the insecticide-susceptible Harlan bed bug strain were placed around the house for on-site neem seed oil vapor toxicity ass ays. Two days after spraying, bed bug mortality from neem seed oil vapors was highest in confined spaces; with 48% mortality in vials placed between the mattress and box spring, versus 28% mortality in open spaces. On Nov. 6, two weeks post-treatment, 123 dead bed bugs were vacuumed up and live bed bugs were detected in a second bedroom.Bed bug numbers were low because the monitoring traps were doing double duty, also providing population suppression by removing many bed bugs. Herbal oils can also be combined with heat chambers at 50 C (122 F) or carbon dioxide (CO2) fumigation chambers to combat bed bugs. However, heat chambers are expensive, and CO2 fumigation with dry ice can pose handling difficulties and room air circulation issues, Dong-Hwan Choe of the University of California, Riverside, told the Entomological Society of America (ESA).Herbal essential oils are useful against head lice, and in Choe’s native Korea clove oil from from the leaves and flower buds of clove plants (Syzygium aromaticum) is used in aromatherapy and as a medicine. Clove oil is rich in GRAS (Generally Recognized as Safe) compounds such as eugenol, beta-caryophyllene and methyl salicylate (sometimes called wintergreen oil), which are useful as vapors in control of insects and microbes. In dentistry, clove oil (eugenol) is widely used as an antiseptic and pain reliever.Clove essential oils work faster in closed spaces or fumigation chambers (e. g. vials, Mason jars) than in open spaces. Essential oils are even slower to kill bed bugs when orally ingested. In experiments at varied temperatures, Choe placed 10 bed bugs in plastic vials with mesh tops. The vials were placed inside 900 ml (1. 9 pint) Mason jars; filter paper treated with essential oils was placed on the underside of the Mason jar tops. Herbal essential oils worked faster at higher temperatures.For example, methyl salicylate fumigant vapors provided 100% bed bug mortality in 30 hours at 26 C (79 F); 10 hours at 3 5 C (95 F); and 8 hours at 40 C (104 F). Eugenol vapors produced similar results; there were no synergistic or additive effects from combining eugenol and methyl salicylate. Choe told the ESA that his future trials will include: botanical oil granules; exposing bed bug-infested items to essential oil vapors; and checking for sublethal essential oil effects on parameters such as female bed bug reproduction.Narinderpal Singh of Rutgers placed bed bugs on cotton fabric squares treated (half left untreated) with synthetic pesticide and herbal essential oil products: 1) Temprid(TM) SC, a mixture of imidacloprid and cyfluthrin (neonicotinoid and pyrethroid insecticides); 2) Ecoraider(TM) (Reneotech, North Bergen, NJ) contains FDA GRAS ingredients labeled as â€Å"made from extracts of multiple traditional herbs that have been used in Asia for hundreds of years for therapy and to repel insects;† 3) Demand(R) CS, which contains lambda-cyhalothrin (a pyrethroid insecticide); 4) Bed Bu g Patrol(R) (Nature’s Innovation, Buford, FL), a mixture with the active ingredients listed as clove oil, peppermint oil and sodium lauryl sulfate. && Temprid(TM) SC and Demand(R) CS proved best on the cotton fabric test. In arena bioassays with Climbup(R)Interceptor traps, none of the four insecticides were repellent to bed bugs (i. e. repellency was less than 30%). Ecoraider(TM) was equal to Temprid(TM) SC and Demand(R) CS against the tough to kill bed bug eggs. Singh concluded that field tests of Ecoraider(TM) as a biopesticide were warranted.Changlu Wang of Rutgers told the ESA that travelers might be protected from bed bug bites and bring home fewer bed bugs if protected by essential oil repellents, as well as by more traditional mosquito and tick repellents like DEET, permethrin and picaridin. Repellents are more convenient and less expensive than non-chemical alternatives such as sleeping under bed bug tents and bandaging yourself in a protective suit. Isolongifolenone , an odorless sesquiterpene found in the South American Tauroniro tree (Humiria balsamifera), is among the botanicals being studied, as it can also be synthesized from turpentine oil and is as effective as DEET against mosquito and tick species.Bed bug arena tests involve putting a band of repellent around a table leg, with a Climbup(R)Interceptor trap below. If the bed bug falls into the trap, it is deemed to have been repelled from the surface above. In actual practice, the bed bug climbs up the surface and goes horizontal onto the treated surface and drops or falls off if the surface is repellent. Isolongifolenone starts losing its repellency after 3 hours; 5%-10% DEET works for about 9 hours. In arena tests with host cues, 25% DEET keeps surfaces repellent to bed bugs for 2 weeks. But isolongifolenone is considered safer, and Wang is testing higher rates in hopes of gettting a full day’s protection. How to Kill Termites: Treatment Options for HomeownersDon’t let th eir size fool you, termites are far from harmless. These small white insects feed on untreated wood piles around homes and can even start up a colony within the structure of your home–where wood is abundant. When termites find their way into homes, they can cause serious structural damage that requires costly repairs. If you’re wondering how to kill termites, contacting a professional to address the problem is the best treatment method you can choose to maintain the integrity of your home. There are different methods you can use to kill termites around your home, but remember that your safest option is to contact a professional to treat your home and property.If you’re waiting for your exterminator to come and inspect your home and you want to be proactive, there are a couple of different treatment options you can try. 1. Boric acid- This white powder is commonly used to kill roaches, but it works with termites as well. You can sprinkle it around the foundation of your home to keep termites from coming in. You’ll need to repeat this treatment every few days for at least two weeks before you notice a decline in the number of termites in your home. 2. Bait blocks-You can also place bait blocks around your home. You can find these in most grocery or hardware stores. These traps contain wood that’s been treated with pesticide.Once the termites find these traps, they’ll carry the poisoned wood back to the queen. Once the queen dies, the termites will be unable to reproduce. 3. Termiticide- If you know the location of the infestation, you can spray the area with a non-repellant termiticide, or you can sprinkle the area with Bio-Blast. Termites that come into contact with pest control products will infect other termites until that infection reaches the queen. However, it may take up to three months before your termite problem is under control. Home treatments can be less expensive than hiring a professional exterminator, but if you don’t treat the problem properly, termite damage can be costly.Your safest option is to contact a professional if you have any suspicion that termites are present. Contacting a professional to treat your termite problem as soon as possible can help you prevent much of this damage and save you from costly repairs. If you have a termite problem, contact one of the pest control experts at Landscaper. org to take care of the problem before it becomes worse. Research Article Termiticidal Activity of Parkia biglobosa (Jacq) Benth Seed Extracts on the Termite Coptotermes intermediusSilvestri (Isoptera: Rhinotermitidae) Bolarinwa Olugbemi Division of Termite Control and Ecology, Termite Research Laboratory, P. M.B. 656, Akure 340001, Nigeria Received 5 October 2011; Revised 14 November 2011; Accepted 28 November 2011 Academic Editor: Arthur G. Appel Copyright  © 2012 Bolarinwa Olugbemi. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract The chemical and mineral composition of raw and boiled seeds of the African locust bean, Parkia biglobosa (Jacq) Benth, was determined while the termiticidal action of the aqueous, alcoholic, and acetone extracts of the bean seeds were investigated.Variations in the proximate and mineral composition of the raw and boiled seeds were obtained while heavy minerals such as cadmium, cobalt, lead, nickel, and copper had been leached out of the seed during the process of boiling. Extracts from the raw seeds exhibited varying degree of termiticidal activity, while extracts from the boiled seed had no effect on the workers of Coptotermes intermedius Silvestri. Alcoholic extracts were more active than the aqueous and acetone extracts. Termites die within 30?min, 40?min, and 110?min when exposed to concentration of 4?g?mL?1 treatments of alcoholic, aqueous, and acetone extracts, respectively. 1. Introduction Termites cause the most serious damage of all wood-feeding insects.In addition to timber and wood products, they attack growing trees, leather, rubber, and wool as well as agricultural crops [1]. Significant damage is caused by termites to man-made fabrics, polythene, plastics, metal foils, books, furniture, wooden telephone poles, wooden railway sweepers, and insulators of electric cables [2]. Damage caused by termites to wooden structures in the United States of America is estimated to be over 3 billion Dollars annually, with subterranean termites accounting for at least 80% of these damages [3]. Costs attributable to Coptotermes formosanus in the Hawaiian Islands alone are greater than 60 million Dollars per annum [4].Termites are so destructive in that they derive their nutrition from wood and other cellulotic materials. In Africa and elsewhere in the developing countries, there is hardly any data on either the quantum of damage d one by termites to agricultural crops, construction timbers, paper, and paper products, or the cost of control or repairing the damage done by these insect pests. The damage done by various termite species in Nigeria [2] ranged from scavenging on tree barks and dead branches, to eating out grooves in the roots and stems of plants. Past research efforts had focused more on chemical methods of control, with an obvious lack of attention placed on understanding the behavior and history of these termites.In view of mounting concerns over the side effect caused by the use of these toxic and environmentally unfriendly chemicals, direction of research is now focusing on alternative nontoxic, biological, and environmentally friendly methods of control. These methods include baiting systems, use of asphyxiant gases, application of extreme temperatures, barriers of various types, as well as biological control organisms [3, 5]. Extractives with insecticidal properties from naturally resistant w ood and plant species in form of phenolic, terpenoid, and flavonoid compounds, show great promise for prevention of termite attack [6–9]. Some of these substances may also act as feeding deterrent [10–12].The termite Coptotermes formosanus was found to be attracted and preferentially feed upon the amino acids, glutamic and aspartic acids [13]. These could be used to improve the effectiveness of baiting systems. Many of the chemicals causing attraction and avoidance in several tree species are polar molecules [14]. Investigation has shown that steaming of the heartwood of the Japanese larch, degraded or removed the chemicals responsible for the inhibition of termite attack [15]. A number of tree species such as the Alaska cedar, redwood, and teak [16] are resistant to termite attack. Neem was found to be a strong repellent to Coptotermes formosanus and was suggested as a barrier tree to protect more vulnerable plants [17].The use of high levels of carbon dioxide, for ex tended period of time has been successfully used to control termites in contained spaces [4]. The application of heated air to kill termites has shown to be successful in laboratory bioassays [18]. Liquid nitrogen has also been shown to be effective in eliminating termites in the laboratory [3]. These temperature-based control methods are showing great promise, but need more field studies on their effectiveness in natural settings. In other studies [19] Inundation with water was shown to cause a decline in foraging worker population. This could indicate possible applications to control, for example, the controlled flooding of the territories of specific termite colonies to reduce damage by foragers.Barriers to foraging termites that are being tested include sand, crushed granite, glass splinters, and metal shields. These methods have had mixed successes, thereby pointing to the need for more research in this area [3]. The African locust bean, Parkia biglobosa (Jacq) Benth, is a pere nnial leguminous tree, found growing wildly in forested and savanna belts in Nigeria. Fermented Parkia seeds are locally used in traditional soup seasoning, medicinal preparations and food additives [20]. In addition, boiled water obtained during fermentation process of P. biglobosa seeds is used in controlling termite infestation at the local level. In spite of this practice, few reports exist on the termiticidal properties of aqueous solution of P. biglobosa seeds.

Physical Dimensions of Human Occupation Essay Example | Topics and Well Written Essays - 750 words

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The Nineteenth Century America Essay Example | Topics and Well Written Essays - 1000 words

The Nineteenth Century America - Essay Example In the year 1819 the Transcontinental Treaty proposed by the then American President John Quincy Adams was signed by the United States and Spain. This treaty reinforced the American belief of territorial expansion. The desire to spread towards the west increased when John Jacob Astor expanded his fur trade company to the Pacific Northwest. With trade expansions the legendary â€Å"mountain men† became popular as they were financially facilitated by the fur trade companies to move to other countries in search of profit. During the 1830s and 1840s the Americans expanded their territory westward towards the Pacific Ocean. Besides economic there were other reasons like avoiding religious persecution. Their attempt to establish American ideals into areas that fell into the boundaries of Mexico or Great Britain created diplomatic crisis. The phrase Manifest Destiny was coined in 1845. It manifested the American belief that God has given them the right to expand their territories bec ause of their growing population. The largest acquisition took place in 1803 when America acquired Louisiana territory for $15 million. With this America got control over Mississippi River and the size of the nation became double. America was greatly affected by the era of expansion. The territorial expansions resulted in dividing the nation by causing diplomatic crisis, war and internal conflicts. The economic expansion that took place internally increased class differences and America began to be recognised as a â€Å"land of opportunity and upward mobility† (Divine et.al, ch.8, 9, 13). II.Transportation Revolution During the first half of nineteenth century America experienced a growth in market economy. This was helped by development in the transportation sector which created an economic link between different parts of the nation and strengthened the national economy. It was the era of expansion in America when the nation expanded its territory towards the West. Most of the land in the east of Mississippi river became marketing centers to fulfill the needs of the farmers. There was a growing awareness among the political leaders regarding the need of connecting these distant areas with the rest of the nation through a well developed transport system. The initial focus was on developing the road network by building new highways and improving the older roads. Overland toll roads or turnpikes could not provide cheap transportation to cover long distances during bad weather because of their poor conditions. More economically efficient was the water transport. Many canals were constructed to estab lish links between the rivers Ohio and Mississippi as canals were cheaper to transport heavy agricultural goods than on poorly maintained roads and highways. The most important canal, the Erie Canal, was constructed in 1825. This canal helped in extending the commercial market to New York and this led to economic development in the region. There were also other improvements made on water transport. Steamboats became popular after they were invented in 1807 by Robert Fulton. (Divine et.al, ch.9) The growth of railroads in the 1840s and the 1850s reduced the importance of canals as primary means of freight transport. The expansion of the railroads helped the iron industry of America. It also facilitated financing of business sectors and paved the way for governments to provide land grants (Divine et.al, ch.13). III. Jackson presidential administrations During the 1820s and 1830s the concept of â€Å"self-made man† was celebrated in the political culture. It promoted the idea th at leaders of the

Own career development Personal Statement Example | Topics and Well Written Essays - 1250 words

Own career development - Personal Statement Example I present in the remainder of this document, my recollections of how I ventured in to my vocation as a Guidance Counselor, comparing my specific case with theories of career development (Career Development, 2003). I conclude with inferences which I use for graduate students who seek my professional help. Informality has marked my progress towards the career. Theories which state that the value of work should be taught in the pre-school years (Career Development, 2003,) and that a relationship has to be made so early in life between education and a career, seem to escape my memory, if at all my parents subscribed to such concepts. This pattern continues as I reflect on my years in school, all the way from kindergarten to when I was ready for college. The post secondary education phase (Career Development, 2003) was an inflexion point for my career. Many of my peers sought vocational training at this time, and only a minority sought college education. We all tried our hands at odd jobs and assignments during this time, but I cannot recall even considering regular employment. This could have been because my family had more than adequate means to provide for my higher education. I do not recall much formal counseling even at this time, though teachers did speak well of my intellectual abilities and potential, and my parents let me know that they would support anything that I wished to do. My choice of college and course was determined largely by peer pressure, though it was all friendly and non-invasive. Adulthood as defined in career development theory (Career Development, 2003) was a defining phase for my career choice. My abilities and weaknesses came in to sharp focus at this time, and I developed a sense of urgency to earn enough to start a family of my own. An older sibling advised me strongly against directions suggested by counselors at college, and I recall being uncertain of which direction to take. I am not yet sure whether I made the right choice for my graduate education, though I am now happily reconciled to my professional situation as a Guidance Counselor. I understand career development theory to advocate a much earlier initiation in to formal planning, and a structured approach to counseling, almost from the inception of cognition (Career Development, 2003). I seem to have escaped with a more carefree child hood, and transited from home to kindergarten and from there to various Grades of school, in almost casual manner! I am not aware that I am much worse off for it, though the career development theory does make a lot of sense when I interact with many graduate students today. Influences Why might I have escaped rather lightly, even though my parents and school teachers did not implement the career development theories of today, during my student days, in structured and formal ways The reasons, on my reflection, seem to lie in strong influences under which I grew up. It may be that my memory is weak and I recall these influences without being able to relate them to formal counseling. Nevertheless, I find my recollections to be useful in understanding how I evolved in to becoming a Guidance Counselor. There were 3 strong influences, which I feel shaped my career choice, though they

Business, Marketing and Finance Coursework Example | Topics and Well Written Essays - 2000 words

Business, Marketing and Finance - Coursework Example A company cannot operate without competent leadership that could provide proper direction of the company using clear vision, mission, goals, and strategies. The human resource or their staffs, regardless if they are few, is the backbone of its business relations and operations to provide customer services or to relate with the market where the business establishment is present. Good leadership & staff Good leader or good staff? This researcher asserts that organizational management require both: good leaders and staff. Both are indispensable in organizational management and development. Good business leadership optimize human resources too for good decision-makings to professionally engage their commitments to attain business objectives and goals. Both the management and the staff are indispensable in contributing to the enrichment of the organization and for its operational management to reach targeted outcome. Its structure will often provide or contribute potential support to comp etitive advantage in business management. Both are stakeholders for organizational development and resource generation. Their cognitive abilities and skills are wealth that can be optimized in systematizing organizational management (Covey, 1992, p. 17) Their indispensable mutual assistance in decision-making will also support the business attain a healthy operational condition (Covey, 1992, p. 17) Good leadership is a principle centered-leadership inspired on personal-trustworthiness, interpersonal relational trust, empowerment-sensitive management, and organizational accountability (Covey, 1992). Covey (1992) contended that good business leaders consider all the natural laws, principles and operational frameworks that put virtues and moral responsibility significant in a person’s life, in relations, in contract management and within the core of the organization (Covey, 1992, p. 17). Covey (1992) asserted that effective and efficient leaders adopt on inviolable principles of practicable human nature that are further nurtured by good norms and culture in a society for better social cohesion. Covey (1992) opined that principles are built and nurtured by society and are imbibed in human condition, as well as, in consciousness. Good leaders prefer to adapt on good value system as guidance and direction for better decision-makings, in planning, in enforcing plans, in resolving conflicts, in managing changes, in valuating performances, and in exercising power in organizational development (Covey, 1992, p. 21). This is in correlation to the fact that an organization is not just a simple structure. Its leader must be a representation of great purpose and targeted outcomes rooted from clear planning and execution of coordinated or collaborated tasks to achieve desired outcomes. Covey’s integrated ethical code on business leadership put principles above interests to holistically nurture organizational development toward for a useful institution of the soc iety. Good transformational leaders are therefore essential as managers that could fully assist in the development of a mutually satisfactory business operation and practice that inspire socially acceptable moral imperatives and standards (Covey, 1992, p. 21). Meager capital? Without enough financial capital, a business cannot commence to operate and manage its supply chain. Effective business leadership knows financial management, although meagre, as essential for organizational management because it permeates stakeholders to make accurate decisions

Planning and Managing for the Future - Inverleith Hotel - Edingburgh Assignment

Planning and Managing for the Future - Inverleith Hotel - Edingburgh - Assignment Example In addition, the managers and employees are identified to be focusing on the preferences of the customers facilitating in attracting more customers, which in turn aids towards the development of the hotel (Annual Report, 2014). One of the weaknesses of hotel is the limited sales volume and number of rooms, which is affecting the visibility of the brand name of the hotel. The major opportunity of the hotel is the competency of the employees based on which the hotel is able to perform its operations in a competitive manner. The entrance of new hotel can be one of the major threats for B&B. The owners and managers play a vital role towards the improvement of the performance of the hotel based on their good managerial skills. The owners and managers always support the employees in their work, so that the employees can perform their work effectively for meeting the satisfaction level of the customers (Annual Report, 2011). In the context, the assignment focuses on evaluating the plans as well as objectives of Inverleith Hotel (B&B) for having a better understanding of the performance of the company. Accordingly measures are needed to be adopted for implementing the changes effectively for enhanced growth as well as development of the hotel. The current business objectives and plans of Inverleith Hotel (B&B) are to increase exposure and expand market by using new and advanced technology. Other current business objective and plan is to increase the number of customers with the aim of increasing the profit as well as economic growth of the hotel. The hotel has planned to improve the quality of food products and also provide the best services to the customers based on the requirements of the customers (Annual Report, 2013). The business plans to hold a strong market position in the international market. The current business objectives and plans have been to enhance the performance of the hotel. To improve the current performance, the owners and

Analysis of Service Learning Essay Example | Topics and Well Written Essays - 500 words

Analysis of Service Learning - Essay Example Habitat for Humanity is a project provided by the non-profit store that creates additional funding to help people who need support in housing purposes. The project was conducted on April 28, 2015, from 1.00 PM to 6.00 pm at the Lynnwood branch and I was a member of the project’s staff. Hence, I helped to tag prices on the incoming items and cleaned the floor in the store. I worked with few people who were great supporters of the project and believed in its success and utility for the society in general and for Snohomish County in particular. I’ve got a lot of experiences concerning trading and teamwork. First of all, I’ve realized that teamwork is a complicated kind communication that requires a lot of patience and ability to conform to other people who one works with. Also, dealing with customers, sometimes I had to be nice and appreciate the position of each and every one of them, so basically I had to understand their needs and the conditions they lived in. Th ough I’ve managed to observe how various are people’s cultural backgrounds, lifestyles, customs, and traditions. It was a great experience that will encourage my further learning of different cultures presented in the United States. The experience I’ve got is definitely closely related to anthropology as I dealt with various kinds of interpersonal communication and integration into multiple forms of American culture. For instance, working with people of various lifestyles and cultural backgrounds made me realize the factors that affect the development of people’s personalities’ and such things as social adaptation, preferences, and even their styles of communication between each other. Hence, exploring different items, both donated and purchased, has made me take a look at people’s various lifestyles and cultures; I also learned how people’s home appliances are related to their social status or striving to get one.

UK MEDICAL LAW Essay Example | Topics and Well Written Essays - 2000 words

UK MEDICAL LAW - Essay Example the fact that medical professionals are deemed ready, willing and able to provide medical treatment and advice and by implication are bound to have the necessary skill and knowledge of a reasonable medical professional.3 A registered medical professional offering his or her service is held to this standard.4 In addition, Lord Brown Wilkinson held in Wilsher v Essex Area Health Authority 1987] Q.B 730, CA [1998] AC 1074 that health authorities owe a duty of care to patients in their care.5 The question then turns on whether or not there was a breach of the standard and duty of care assigned to the medical profession on the part of Doctor Greene and/or Wilington hospital.. In determining whether or not there was a breach of the duty and standard of care, the Bolam test is applied. In Bolam v Friem Hospital Management Committee [1957] 1 WLR 582 the court devised a two-tier test to determine whether or not the medical professional or institution breached the applicable standard and duty of care.6 The first leg of the test inquires into whether or not the defendant professional acted in a manner that corresponds with the reasonable skilled person or vocation of the profession at issue.7 The second leg of the test inquires into whether or not the treatment or advice administered was consisted with a body of opinion in the profession.8 In ascertaining whether or not Doctor Greene, acting on behalf of and on the authority of the hospital complied with the Bolam standard of care, the court will look to the circumstances in which the treatment was administered and the attending physician’s post or position.9 On the facts of the case for discussion there is no specific evidence of Dr. Green’s position or post within the hospital, except that he is from the casualty department. The question then turns on whether or not a doctor in the emergency room who specializes in the treatment of children would have taken the action that Dr. Green took. Dr. Green

Questions on Physics Essay Example for Free

Questions on Physics Essay What happens to the strength of an electric field when the charge on the test charge is halved? 6. What happens to the electric potential energy of a charged particle in an electric field when the particle is released and free to move? 7. A test charge experiences a force on . 30N on it when it is placed in an electric field intensity of 4. 5 x 10? N/C. What is the magnitude of the charge? Q= 6. 7 x 10-7C 8. What is the electric field strength 20. 0 cm from a point charge of 8. 0 x 10 C? E=1. 8X105 N/C 9. How much work is done to transfer 0. 15C of charge through an electric potential difference of 9. V? W=1. 35J 10. A voltmeter measures the electric potential difference between two parallel plates to be 60. 0 V when the plates are separated by 3. 0 cm. What is the electric field strength between the plates? 11. Describe two ways to increase the current in a circuit. 12. Sue finds a device that look like a resistor. When she connects it to a 1. 5V battery, she measures only 45 x 10 A, but when she uses a 3. 0 V battery, she measures 25 x 10 A. Does the device obey Ohm’s law? NO 13. Refer to the diagram below to answer the following questions. a. What should the ammeter reading be? 1. 5 A b. What should the voltmeter reading be? 27V c. How much power is delivered to the resistor? 40. 5 W d. How much energy is delivered to the resistor per hour? 1. 46105 J/hr. 14. For each of the following write the form of circuit that applies: series or parallel. e. The current is the same everywhere throughout the entire circuit. f. The total resistance is equal to the sum of the individual resistance. g. The voltage drop across each resistor in the circuit is the same. h. The voltage drop in the circuit is proportional to the resistance. i. Adding a resistor to the circuit decreases the total resistance. j. The voltage drop to the circuit increases the total resistance. k. If the current through one resistor in the circuit goes to zero, there is no current in the entire circuit. l. If the current through one resistor in the current goes to zero, the current through all the other resistors remains the same. m. This form is suitable for house wiring. 15. The load across a battery consists of two resistors with values of 15 ohms and 47 ohms, connected in series. n. What is the total resistance of the load? 62? o. What is the voltage of the battery if the current in the circuit is 97mA? 6. 0V 16. A 16. 0 ohm and a 20. ohm resistor are connected in parallel. A difference in potential of 40. 0 V is applied to the combination. p. Compute the equivalent resistance of the parallel circuit. 8. 9? q. What is the total current in the circuit? 4. 5V r. What is the current in the 16. 0ohm resistor? 2. 5 A 17. Describe how a permanent magnet differs from a temporary magnet. 18. A wire that is 0. 50m a nd carrying a current of 8. 0A is at a right angle to a uniform magnetic field. The force on the wire is . 40N. What is the strength of the magnetic field? B=0. 1 T 19. A beam of electrons moves at right angles to a magnetic field of 6. x 10 T. The electrons have a velocity of 2. 5 x 10? m/s. What is the magnitude of the force on each electron? F=2. 410-14 N 20. An electric wire inside the wall of a building carries a dc current of 25 A vertically upward. What is the magnetic field due to this current at a point10 cm due north of the wire? B=5. 0x10-5T 21. Describe interference. Is interference a property of only some types of waves or all types of waves? 22. What happens to a spring at the nodes of standing waves? 23. Water waves in a lake travel 3. 4m in 1. 8s. The period of oscillation in 1. 1s. s. What is the speed of the water waves? 1. 9m/s t. What is their wavelength? 2. 1m 24. The frequency of yellow lights is 5. 1 x 10 Hz. Find the wavelength of yellow light. The speed of light is 3. 0 x 10? m/s. 5. 910-7 m 25. If the pitch of sound is increases, what are the changes in the following? u. the frequency v. the wavelength w. the wave velocity x. the amplitude of the wave 26. The sound from a trumpet travels at 351m/s in air. If the frequency of the note is 298Hz, what is the wavelength of the sound wave? 1. 18m 27. A ray of light strikes a mirror at an angle of 38Â ° to the normal. What is the angle that the reflected angle makes with the normal? 38o 28. A ray of light incident upon a mirror makes an angle of 36Â ° with the mirror. What is the angle between the incident ray and the reflected ray? 108o 29. An object is placed 15 cm from a converging lens with a focal length of 10 cm. Determine the image location. Draw the ray diagram for this situation and describe the image. d i = 30. 0 cm 30. An object is 36. 0 cm in front of a concave mirror with a 16. 0 cm focal length. Determine the image position. Draw the ray diagram for this situation and describe the image d i = 28. 8cm

Robert Browning Essay Example for Free

Robert Browning Essay The dramatic monologue form which is now widely used, allows the author to engage his reader more directly by placing him in the role of listener. Often they are to interpret about a dramatic event or experience they are reading about. This allows the reader to become more intimate with the writer and the characters while being able to understand the speakers changing thoughts and feelings. This is almost like being inside the mind of the speaker not the writer though. The speech is delivered by a character in a play allowing the imagination of the reader to remain open. Many poets have used some type of dramatic monologue forms in their poetry but none as much or as skilled as the Victorian poet Robert Browning. Even though some believe the works of Robert Browning in fact did not invent the dramatic monologue form and argue that dramatic monologue was used in poetry preceding Browning I believe he set the standard for the form. Robert Browning is considered the master of the dramatic monologue because Browning’s use of dramatic monologue changed post romantic poetry and the dramatic monologue technique. Browning’s dramatic monologue writings were the first to achieve its distinction. I think initially some believed that some dramatic lyrical poems took the form of dramatic monologue it was more of reading the views of the poet and putting the reader into the mind of the actual poet verses putting the reader into the mind of the character. They key to dramatic monologue is the poet is telling a story through the characters of the story and the views of the characters are not the same views the poet would have if it were written in a different form. It wasn’t until realizing that these other poems in fact did not follow the same techniques of Robert Browning’s work that Browning received the recognition for being the inventor of dramatic monologue poetic form. Browning is now considered the master of the dramatic monologue. One of his most recognized dramatic monologue form poems is My Last Duchess. His careful choice in words, skillful technique and ability to leave some information up to the reader to interpret gave him his distinction and effectively named him the master of the dramatic monologue form. The poem My Last Duchess is about a powerful Duke and his beautiful deceased wife. The poem is based during the Renaissance years, in Italy, and revolves around the Duke of Ferrara. The Duke has either a visitor or servant that he is talking to. It is believed that he is planning his next marriage and discussing this with his guest. They pass a portrait of his previous wife that is painted on the wall and he stops to reminisce. The Duke appeared to truly love his duchess and this was evident by having her memory displayed as a portrait on his wall for all to see. The poem begins with him mourning her loss and I believe ends with the justification of her death. The Duke felt she behaved in a manner that was unbecoming of a duchess. The Duke believed that she was a cheater and this caused him embarrassment and anger. He stated she was a kind hearted and a joyful woman, the same qualities that initially attracted the Duke to the Duchess. The Duke stated that her smiling and flirting with other men and the fact that she was not trying to hide what she was doing was unacceptable and shameful. These same qualities would eventually lead to her demise The Duke was furious and Browning’s use of dramatic monologue shows us it’s not exactly what the Duke says to the visitor about his Duchesses death but what he indirectly reveals by not telling the whole story and leaving it up to the reader to decide. The fact that there is a lot not said in this story I believe shows his fury, the way he spoke of her could lead one to believe that he either killed her or had her killed. It seems back in those days it was common for wives to be killed as divorce was not accepted. This is just one small sample of Browning’s work. Browning’s fame today rests mainly on his dramatic monologues, in which the words not only convey setting and action but also reveal the speaker’s character.

Growth Characteristics of Bacteriophage

Growth Characteristics of Bacteriophage CHAPTER 4 GROWTH CHARACTERISTICS OF BACTERIOPHAGE INFECTING AQUACULTURE BACTERIAL PATHOGENS 4.1  Introduction Bacteriophage are naturally occurring viruses that predated on bacteria (Clokie et al., 2011). They self-replicate exponentially and leave the commensal flora unaffected makes them useful for industrial application (Tsonos et al., 2014). However, the high number of bacteriophage in environment (Clokie et al., 2011) provide the challenges to the discovery of the most effective phage in treating bacterial pathogens (Lindberg et al., 2014). Even there were many extensive reports on bacteriophages, the clinical outcome of therapy trials are variable (Tsonos et al., 2014). This indicates that there are still many parameters which are unclear that may contributed to efficacy of the phage therapy. Previously, the most common practice to evaluate the therapeutic efficacy of phages was from in vivo studies. However, Lindberg et al. (2014) provide the alternative to evaluate the efficacy the phage treatment. The information could be assessed from the important phage traits such as adsorption, lysis time and burst size (Ackermann et al., 2004). Besides that, there are various physical and chemical factors like temperature, pH and salinity which could determine the occurrence and stability of bacteriophage (JoÅ„czyk et al., 2011). These factors reported to cause the inactivation of phage through damage of the phage structure (head, tail or envelope) or DNA structural changes (Ackermann et al., 2004). Therefore, the next section in this study is aimed to characterize the bacteriophage isolates (VALLPKK3, VHLPKM4 and VPLPKK5) based on their adsorption profile, one step growth profile and stability to various range of temperature, pH and bile salt concentration. 4.2  Materials and Methods 4.2.1  Bacteriophage Isolates The bacteriophage isolates that were used in the third chapter were further characterized in this chapter. The bacteriophage isolates were designated as VALLPKK3, VHLPKM4 and VPLPKK5. 4.2.2  Bacteriophage Adsorption Assay The bacteriophage adsorption assay was carried out following the method described by Hsieh et al. (2011) with few modification. In adsorption test, the host bacteria was first grown to OD600 1.0 or equivalent to ~108 cfu/ml and diluted to ~105 cfu/ml with TSB media. About nine ml of the host bacteria was mixed with one ml of phage lysate (~103 pfu/ml) to MOI of 0.001. Then, 100  µl of the bacteria-phage mixture was taken to determine the initial phage titer. The mixture was then incubated at 28 °C with no agitation. After 10 min, one ml of the samples was collected and centrifuged at 16,000 xg for 2 min to precipitate the absorbed phages. The same was repeated every 10 min for a period 50 min. The count of unabsorbed free phages in the supernatant was determined. Then, the free phage particles over the initial phage particles was calculated and expressed in percentage. The accuracy of the free phage count was improved by triplicate separate experiments. 4.2.3  Bacteriophage One Step Growth The one step growth assay was determined following method described Hsieh et al. (2011) with slight modification. First, host bacteria (OD600 1.0) was diluted to ~106 cfu/ml. Then, 100  µl of bacterial suspension was mixed with 100  µl of phage lysate (~103 pfu/ml) to a 1 ml of final volume with sterile TSB media (MOI 0.001). Second, the phage was allowed to adsorb into bacterial cells for 30 min at 28 °C. Then the bacterial cells were precipitated by centrifugation at 16,000 xg for 2 min. Third, the bacterial-phage pellet was suspended in 50 ml sterile TSB. Subsequently, 1 ml of the bacterial-phage suspension was precipitated by centrifugation and plated to determine the initial phage count. Then, two sets of bacterial-phage suspension concurrently collected every 12 min for a period of 84 min (for VALLPKK3 and VPLPKK5) and 132 minutes (VHLPKM4) for the determination of latent period, eclipsed period and burst size. The first set was subjected to above treatment to determine t he latent period and burst size while the second set was added with 40  µl chloroform, mixed and incubated at 28 °C for 5 min before centrifugation to determine the eclipse period. The free phage count in the supernatant was determined in triplicate. The latent period and burst size was determined according to Middleboe et al. (2010). The eclipse period was determine according to Sillankorva et al. (2008). The accuracy of the free phage count was improved by triplicate separate experiments. 4.2.4  Bacteriophage Tolerance Test The stability of the bacteriophage isolates was test in different range of temperature, pH and bile salt concentrations. The temperature test was conducted for one hour, while, the pH and bile salt concentration tests were conducted for 24 hours. a.  Temperature Tolerance Test The stability of bacteriophage in different temperature was done following method described by Phumkhachorn and Rattanachaikunsopon (2010) with slight modification. The bacteriophage solution was set to approximately 105 pfu ml-1 in sterile phage buffer. About 900  µl of sterile phage buffer was distributed into sterile empty 1.5 ml microfuge tube. The tube was incubated in the dry bath at desirable temperature (40, 50, 60, 70, 80, 90 and 100 °C) at least for 30 minutes. After 30 minutes, about 100  µl of bacteriophage solution (~104 pfu) was added into the preheated tube and mixed immediately. The tube was incubated again at desirable temperature for an hour. After incubation, the tube was placed in ice-warm bath to cool the bacteriophage solution. The titer of the survival phage was calculated by double layer method. The percentage of surviving phage was calculated by dividing the number of survival phage over initial phage count. b.  pH Tolerance Test The stability of bacteriophage in different pH was done following method described by Hsieh et al. (2011) with slight modification. The pH of phage buffer was adjusted into desirable pH (2, 3, 4, 5, 6, 7, 8 and 9) using pH meter (brand). The phage buffer was sterilized using autoclave machine at 121 °C for 15 minutes. The bacteriophage was set to approximately 107 pfu ml-1 in sterile phage buffer. The bacteriophage suspension was diluted to 105 pfu ml-1 (1/100) in phage buffer with different pH. The initial phage count was calculated and the bacteriophage solution was incubated at room temperature for 24 hours. After incubation, the bacteriophage solution was diluted using normal phage buffer and plating to calculate the survival phage by double layer method. The percentage of surviving phage was calculated by dividing the number of survival phage over initial phage count. c.  Bile Salt Tolerance Test The stability of bacteriophage in different bile salt concentration was done following method described by previous. The stock of bile salt (Brand) in phage buffer was prepared by filter sterilized to final concentration of 5 %. Then, the phage buffer was adjusted into desirable bile concentration (5000 ppm, 6000 ppm, 7000 ppm, 8000 ppm and 9000 pm). The phage buffer which used for the dilution of bile concentration was presterilized using autoclave machine at 121 °C for 15 minutes. The bacteriophage was set to approximately 107 pfu ml-1 in sterile phage buffer. The bacteriophage suspension was diluted to 105 pfu ml-1 (1/100) in phage buffer with different bile concentration. The initial phage count was calculated by serial dilution in normal phage buffer. The treated bacteriophage solution was incubated at room temperature for 24 hours. After incubation, the bacteriophage solution was diluted again using normal phage buffer and plated to calculate the survival phage by double laye r method. The percentage of surviving phage was calculated by dividing the number of survival phage over initial phage count. 4.3  Result 4.3.1  Bacteriophage Adsorption Assay In the adsorption analysis, all isolates have two adsorption phases, rapid and slow adsorptions. The rapid adsorption of VALLPKK3 was occurred within 10 minutes where almost 80% of the phage adsorb to the host (Figure 4.1). This result was similar to the VHLPKM4 (Figure 4.2). Meanwhile, the rapid adsorption of VPLPKK5 showed that around 60 % of the phage adsorbed to the host (Figure 4.3). After 10 minutes, the slow rate was occurred to all isolates. The number of unadsorbed phages was approximately below 20% within 40 minutes in all phages. The increase of phage count in VPLPKK5 was occurred after 40 minutes. The increase in free phages after 50 minutes indicates that the newly formed phages are being release from the infected cells (Figure 4.3). Figure 4.1: Adsorption of VALLPKK3 to V. alginolyticus ATCC ® 17749TM Figure 4.2: Adsorption of VHLPKM4 to V. harveyi VHJR7 Figure 4.3: Adsorption of VPLPKK5 to V. parahaemolyticus VPHG1 4.3.2Bacteriophage One Step Growth The one step growth was performed to identify different phases of the phage infection process. During the initial stage, the phage-bacteria cell was separated from the free phage during the adsorption since the adsorption result showed the availability of free phage after 30 minutes of incubation. After the infection, the phage growth parameters (latent period, eclipse period and burst size) were determine from the average of three independent curves. The analysis showed that the latency and eclipse periods of VALLPKK3 (Figure 4.4), VHLPKM4 (Figure 4.5) and VPLPKK5 (Figure 4.6) were 48 and 36 minutes, 60 and 36 minutes and, 36 and 24 minutes, respectively. The latent period of VHLPKM4 was longer compared to VALLPKK3 and VPLPKK5. Meanwhile, the eclipse period of VALLPKK3 and VHLPKM4 was similar, while, the eclipse period of VPLPKK5 were shorter than those two isolates. The VALLPKK3, VHLPKM4 and VPLPKK5 showed a burst size of ~174, ~52 and ~180 phage per infected cell, respectively, at the 28 °C. Figure 4.4: One step growth curve of VALLPKK3 infected with Vibrio alginolyticus ATCC ® 17749TM at MOI of 0.001. The number of PFU per infected cell in untreated culture () and chloroform-treated culture () are also shown. The burst size, latent period and eclipse are indicated as B, L and E, respectively. Figure 4.5: One step growth curve of VALLPKK3 infected with Vibrio harveyi VHJR7 at MOI of 0.001. The number of PFU per infected cell in untreated culture () and chloroform-treated culture () are also shown. The burst size, latent period and eclipse are indicated as B, L and E, respectively. Figure 4.6: One step growth curve of VPLPKK5 infected with V. parahaemolyticus VPHG1 at MOI of 0.001. The number of PFU per infected cell in untreated culture () and chloroform-treated culture () are also shown. The burst size, latent period and eclipse are indicated as B, L and E, respectively. 4.3.3  Bacteriophage Tolerance Test The activity of all phage isolates was stable at 40 °C and declined at 50 °C following heating for 60 minutes. The activity was disappeared entirely when heated at more than 60 °C for 1 hour (Figure 4.7). When compared among the isolates, the activity of VHLPKM4 were decline dramatically to less than 20 % when incubated at 50 °C. The activity of VALLPKK3 and VPLPKK5 were dropped to 80% and 40%, respectively. The activity of bacteriophages VALLPKK3, VHLPKM4 and VPLPKK5 can be measured after incubation at pH 4 to pH 9, but disappear completely at pH 2 and pH 3 (Figure 4.8). When compared among isolates, the VALLPKK3 was sensitive to wide range of pH. Almost all of the VALLPKK3 activity was drop to 20 to 40 % after 24 hours incubation. Meanwhile, the activity of VHLPKM4 was decline to 60 % at pH 4 and 5, relatively stable at pH 6 to pH 8 and decline again to less than 60 % at pH 9. However, the activity of VPLPKK5 relatively stable at wide range of pH (pH 4 to pH 9). Meanwhile, the activity of VALLPKK3, VHLPKM4 and VPLPKK5 can be detected after incubated at bile salt concentration from 5000 ppm to 9000 ppm (Figure 4.9). Among the isolates, VALLPKK3 was more sensitive to the bile compared to VHLPKM4 and VPLPKK5. Figure 4.7:The temperature stability of VALLPKK3, VHLPKM4 and VPLPKK5. All isolates were incubated at various range of temperature (40 °C, 50 °C, 60 °C, 70 °C, 80 °C, 90 °C and 100 °C) for 1 hour. Data are the means from three independent experiments + SD. Figure 4.8:The temperature stability of VALLPKK3, VHLPKM4 and VPLPKK5. All isolates were incubated at various range of pH (2, 3, 4, 5, 6, 7, 8 and 9) for 24 hours. Data are the means from three independent experiments + SD. Figure 4.9:The bile salt stability of VALLPKK3, VHLPKM4 and VPLPKK5. All isolates were incubated at various range of bile salt concentration (5000, 6000, 7000, 8000 and 9000 ppm) for 24 hours. Data are the means from three independent experiments + SD. 4.4  Discussion The phage adsorption of VALLPKK3 and VHLPKM4 was fast (more than 80% after 10 minutes) compared to Vibrio phage PW2 (60% after 10 minutes) (Phumkhachorn and Rattanachaikunsopon, 2010). Meanwhile, the adsorption of VPLPKK5 was comparable to PW2. This might due to both phages were belonged to same family (Sipboviridae). However, the phage adsorption was reported dependent on various condition. According to Binetti et al. (2002), the phage adsorption was shown to be affected by the presence of ion calcium, physiological state of the cell, pH and temperature. The one step growth is a method to assess the life cycle of the phage (Middleboe et al., (2010). The latent period was the time from adsorption to the release of new progeny from host cell, and the burst size was the number of new virus particles liberated from a single bacterial cell (Bao et al., 2011). When compared to other vibriophage infecting same host species, there were difference in term of the burst size of the phages Ñ„As51 and Ñ„A318 (Liu et al., 2014). The V. alginolyticus phage VALLPKK3 showed high burst size compared to those two (72 and 10 PFU/infected cell). Similar finding with VPLPKK5. This V. parahaemolyticus phage was different to other V. parahaemolyticus phage VP-2 (15 PFU per infected cell) (Silva et al., 2014) where it showed bigger burst size (180 PFU per infected cell). Meanwhile, the VHLPKM4 showed different finding. This study showed smaller burst size and longer latent period compared to previous report on V. harveyi phages цžH17-7b and Ñ„H17-8b (Okano et al., 2007). They reported that where the latent period and burst size of Ñ„H17-7b and Ñ„H17-8b were 35 minutes and 100 particles, and 40 minutes and 170 particles, respectively. Thus, the findings showed that the life cycle of each phage isolates was different from each other. However, the significant of the differences was unclear since the dissimilarity was influenced by the host, medium, temperature and its own growth rate (Carey-Smith et al., 2006). In this study, the bacteriophages VALLPKK3 and VPLPKK5 showed a short period of latent period and large burst size. The shorter latent period and large burst size showed that the bacteriophages replicated more quickly and the new virus particle release more efficiently (Bao et al., 2011). This characteristic showed good candidacy of phage therapy (Silva et al., 2014). Finally, both adsorption and one step growth of phage are important to determine the phage fitness (Wang, 2006) since the phage fitness would determine the efficacy of the phage therapy (Lindberg et al., 2014). The stability in various stress condition were useful for the application of bacteriophage to inhibit the target bacteria (Lee et al., 2014; Krasowska et al., in press). In this study, the resistance to heat, pH and bile was investigated to determine the efficacy of those phages for biocontrol of V. alginolyticus, V. harveyi and V. parahaemolyticus infections. Phage which can withstand various environmental stress may be useful for the application in aquaculture (Phumkhachorn and Rattanachaikunsopon, 2010). The temperature is a important factor that affects bacteriophage survivability (Olson et al., 2004). It plays important roles in the bacteriophage attachment, penetration and multiplication (JoÅ„czyk et al., 2011). In this study, the result showed that all phages were stable at 40 °C. However, the viability was reduced after one hour incubation at 50 °C. All phage were completely inactivated in temperature over 60 °C. The phage in this study showed that they are sensitive to high temperature. This findings was different to the findings by Phumkhachorn and Rattanachaikunsopon (2010) where the phage can withstand high temperature. However, in the natural environment, the temperature usually fluctuated at the range of 28 to 32 °C (Albert and Ransangan, 2013). Since the isolates in this study were stable at the temperature up to 40 °C, the isolates would survive when release to natural environment. Nevertheless, the period of viability of these isolates after release to natural environment was unknown. In the natural environment, the phage was also facing the other stress factor such as pH. According to Krasowska et al. (in press), the acidity and alkalinity of environment are other important factors influencing phage stability. It was also reported that low pH influences phage aggregation and reduce their adsorption on bacteria cell (Langlet et al., 2007). Therefore, it was important to access the stability of the current phage isolates in different pH. The VALLPKK3 and VHLPKM4 showed resistance to acid (pH 4) and alkaline (pH 9). This showed that the member of Myoviridae family stable at acid and alkaline condition (Krasowska et al., in press). Similar to the other isolates, VPLPKK5 was also showed resistance to acid and alkaline condition. This is similar to the finding by Lasobras et al. (60) where the member of family Siphoviridae were most resistant to adverse conditions. However, this finding was different to phage ARà ¯Ã¢â€š ¬Ã‚ , a member of Siphoviridae, which is only a ctive in a narrow pH range (Krasowska et al., in press). The result of the phage tolerance to pH indicated that they were tolerant to wide range of pH. In aquaculture, oral administration was the most practical delivery method for immunization (Yasumoto et al., 2006) due to low cost and less stress to fish (Pal et al., 2009). However, the viability of orally administered phage might be rapidly reduced the presence other digestive compounds such as bile (Joerger et al., 2003). In this study, the phage isolates were exposed to various concentration of bile concentrations and result showed that the phage were still survived after incubation. However, there were reduction on the viability of the phage isolates which might showed the adverse effect of bile. With the addition of pH and other enzymes, the phage might not persist for long time in gut environment (Ma et al., 2008). 4.5  Conclusion In summary, the VALLPKK3, VHLPKM4 and VPLPKK5 were characterized by the growth and tolerance. The life cycle of the current isolates might be different when conducted different time and with different media. Therefore, the optimization was required for optimum phage multiplication which generally required for large scale production. This optimization was also contributed to the development of phage therapy. All phages are inactivated at high temperature but showed stability at temperature 40 °C. They are also stable at wide range of pH but not low pH. But, they could tolerate normal fish bile content. However, the study need to be conducted to collect the information of the period of phage survival in fish body. This information would be beneficial for the phage administration of disease treatment.

A Gradual Decline in Prejudice between Places and People in North and S

A Gradual Decline in Prejudice between Places and People in North and South Through her characterizations of the two main characters in Margaret Hale and John Thornton, Elizabeth Gaskell develops a transition from prejudice to love. She reveals their inner most thoughts through an omniscient third person narrative to allow the audience to empathise with their feelings. The Characters develop through dialogue because they dispute over the North and South divide and try to distance themselves from each other. But despite their prejudices love prevails because of the actions of good will shown by Margaret, which John interprets to be feelings of love. The audience is satisfied that they have found a general cause which is sufficient to counteract the forms of prejudice depicted in the novel, and that cause is love. The fundamental theme of prejudice in the story is location. Gaskell contrasts the North and South almost as if they were two entirely different countries. Helstone is a sunny place, and the days are lazy and care free for Margaret. As they approach Milton ...

Ecstasy :: essays research papers

Ecstasy is a very popular designer drug. It is said to give complete euphoria for up to 6 hours. It came around in the 80’s and is becoming more popular now. It’s real name is MDMA (3,4-Methylenedioxy-N-Methylamphetamine). It is a dangerous high and is more popular with young people. In the following paragraphs are some information about the drug. Ecstasy is sold on the streets and is illegal as a schedule 1 drug, along with heroin and cocaine. It has many street names like beans, X, E, rolls, etc... Ecstasy can range anywhere from $5 to $25 and is usually a 100-150 mg pill. It’s known as the â€Å"love drug† because you are supposed to be very generous and friendly when on the drug. Effects generally happen within 20-40 minutes, when the user often experiences a brief "rush" of energy, usually described as mild but euphoric. After this rush, the high levels off to a peak which lasts 2-3 hours and is followed by a "coming down" sensation. MDMA exerts amphetamine-like effects which include dilated pupils, dry mouth and throat, tension in the lower jaw, grinding of the teeth, nystagmus (eye wiggles), sweating, or nausea and overall stimulation. These side effects are dose dependent and will vary depending on the health of the individual user. The following are some feelings or emotions the ecstasy user experiences: Entactogenesis ("touching within") This is a generalized feeling that all is right and good with the world. People on MDMA often describe feeling "at peace" or experiencing a generalized "happy" feeling. Also, common everyday things may seem to be abnormally beautiful or interesting. Empathogenesis Empathogenesis is a feeling of emotional closeness to others (and to one's self) coupled with a breakdown of personal communication barriers. People on MDMA report feeling much more at ease talking to others and that any hang-ups that one may have with regard to "opening up" to others may be reduced or even eliminated. An enhancement of the senses MDMA can significantly enhance (sometimes distort) the senses - touch, proprioception, vision, taste, smell. â€Å"Beaners† can sometimes be seen running their hands over differently textured objects repeatedly, tasting and smelling various foods/drinks. â€Å"Ecstasy. The perfect word. No other could have described what I was experiencing right then and there. Beyond words. The entire message was contained in a scream and the most intense smile my face had displayed in ages.